It is an undershrub climber with extensively branched woody stems, growing up to 2m in height. Asparagus racemosus, pharmacognosy, pharmacology, phytochemistry, shatavarins 1. The plant kingdom represents an enormous reservoir of. Asparagus racemosus shatavari is a plant with immense medicinal values. Micropropagation of asparagus racemosus shatavari abstract.
Shatavari is in the wild asparagus family, hence its botanical name asparagus racemosus. This plant possesses a wide range of secondary metabolites inclusive of steroids, alkaloids, dihydrophenanthrene. Little work has been undertaken on in vitro culture in asparagus racemosus as compared to that of a. According to the website of american diabetes association type 2 diabetes is the most common form of diabetes, in type 2 diabetes, either the body does not produce enough insulin or the cells ignore. Randomized controlled trial of asparagus racemosus shatavari as a lactogogue in lactational inadequacy. It is a beautiful plant with wispy, graceful stems and branches, thin, needlelike leaves, and delicate flowers. Request pdf in vitro propagation of a high value medicinal plant.
Survival of cultured cells and somatic embryo of asparagus pdf. Liliaceae, commonly known as satawari hindi is a perennial shrub, with a tuberous rootstock, stems covered with recurved spines, linear leaves arranged in a tuft, white flowers and sweetscented appears in october. This study was initiated to optimize the conditions for production of shatavarins in cell cultures of a. In the present study, somatic embryogenesis via zygotic embryos was studied in asparagus racemosus willd. The present study on micropropagation of asparagus racemosus through nodal. Pdf micropropagation and phytochemical analysis of asparagus. Asparagus racemosus and crataeva magna remain species with tremendous potential and although considerable work has been done to exploit the biological activity and medicinal applications of these plants, countless possibilities for investigation still remain in relatively newer areas of its function. Asparagus racemosus, traditionally known as shatavari means who medicines, lists asparagus racemosus as part of thomson m. World health organization who estimates that the holistic system is gaining more popularity. Shatavarin iv is a glycoside of sarasapogenin having two molecules of rhamnose and one molecule of glucose 2, 3.
Neet biology strategies for enhancement in food production. Shatavari asparagus racemosus is a powerful therapeutic agent, which is of great importance in ayurveda. Asparagus racemosus is the one most commonly used in traditional medicine 1. Leaves contain 12 withanolides, alkaloids, glycosides, glucose and free amino acids. Active ingredients of asparagus racemosus are believed to be four saponins shatavarin i to iv. Jan 17, 2017 now a day ayurvedic products are suffering from a serious problem of adulteration with addition of low grade, spoiled, inferior, spurious drugs, and useless parts of same or different plant, harmful substances or totally different drug either free from or inferior in therapeutic and chemical properties from original drug which do not confirm.
It is an extensively scandent spinous, much branched undershrub. M in the medium completely inhibited the growth and. But its what is below ground that has captured and held the reverence of ayurveda for the past few millennia. Asparagus racemosus is a commercially important medicinal plant, traditionally used for combating gynecological problems in india. Ms, full strength of ms basal medium supplemented with 3 and 6% sucrose and white root culture medium. Shatavari, or asparagus racemosus, has been used for centuries in ayurveda to support the reproductive system, particularly for females, and as a support for the digestive system, especially in cases of excess pitta. The therapeutic applications of this plant have been reported in indian and british pharmacopoeias and in traditional system of medicine, such as ayurveda, unani and siddha. Berries contain a milk coagulating enzyme, two esterases, free amino acids, fatty oil, essential oil and alkaloids. Study of phytohormones effect on micropropagation and shoot induction in asparagus racemosus int j cur res rev, nov 2012 vol 04 22 page 48 in case of reproductive disease. Since the callus quality plays an important role in plantlet development, therefore, compact embryogenic callus was selected for further embryogenesis.
Translated as having one hundred roots and also referred to as meaning having one hundred husbands, 1, 2 shatavaris name gives reference to its traditional use as a. The present study on micropropagation of asparagus racemosus through. This study was carried out to establish an efficient in vitro propagation protocol. The roots are said to be tonic and diuretic and galactgogue, the drug has ulcer healing effect probably via strenthening the mucosal resistance or cytoprotection. By in vitro propagation of asparagus, root initiation is difficult. C regenerated shoots on sem with 1 mg l1 bap and 0. Asparagus racemosus is a perennial climber climbs upto m high. Micropropagation of asparagus densiflorus via axillary. In vitro plantlet regeneration in asparagus racemosus. Its medicinal usage has been reported in the indian and british pharmacopoeias and in traditional systems of medicine such as ayurveda, unani, and siddha. Asparagus racemosusethnopharmacological evaluation and.
Roots numerous fusiform, succulent and tuberous with a diameter of 0. Notes, study material, practice question and answers, online tests,download pdf. In vitro micro propagation of soybean glycine max bari5. This site is like a library, you could find million book. Micropropagation and phytochemical analysis of asparagus. The explants were cultured on murashige and skoog ms medium supplemented with 6benzyladenine ba, thidiazuron tdz andor. Conservation of medicinal plant asparagus racemosus. It is good source of folic acid, potassium, dietary fiber.
Protocols for in vitro cultures and secondary metabolite. Micropropagation of ringal bamboo drepanostachyum falcatum nees keng f. The genus is considered to be medicinally important because of the presence of steroidal saponins and sapogenins in various parts of the plant. Micropropagation and phytochemical analysis of asparagus racemosus was investigated in the present study. Most of the cultures in hormone media induced callus in the beginning but ms hormone free media showed no response of any kind in callus induction with all the. The optimal medium for asparagus micropropagation described in this. Liliaceae is a pharmacologically acclaimed phytoestrogenic medicinal plant used for its immunomodulatory diwanay et al. Various plant parts are consumed in a variety of ways, viz. Moshe reuveni, aro, volcani center, dalia evenor, and mike timko.
Oct 14, 2015 in vitro seed germination and micropropagation of primrose primula heterochroma stapf. The genus asparagus includes about 300 species around the world. The multiple uses of this species have increased its commercial demand resulting in overexploitation. Asparagus racemosus is a perennial growing to 7 m 23ft. This plant possesses a wide range of secondary metabolites inclusive of steroids, alkaloids, dihydrophenanthrene derivatives, flavonoids, furan. Effect of feeding shatavari asparagus racemosus root. The species is hermaphrodite has both male and female organs and is pollinated by bees. Asparagus racemosus leaf extract inhibits growth of uok. Asparagus racemosus wild locally known as satawar belongs to the family liliaceae. The plant with immense medicinal potential lakhwinder singh, antul kumar, anuj choudhary and gurwinder singh abstract the genus asparagus belonging to the family liliaceae. Rapid in vitro micro propagation of asparagus racemosus willd. Plants are valuable sources of a vast array of chemical compounds like alkaloids, flavonoids, pigments, antimicrobials, pharmaceutical, etc.
Asparagus racemosus satavar, shatavari, or shatamull, shatawari is a species of asparagus common throughout india and the himalayas. Asparagus racemosus is usually propagated by planting the separated tuberous roots along with shoot apex. It will also aid the immune system when antibodies are fighting off a threat. The major obstacle of in vitro micropropagation of asparagus was not the establishment of shoot multiplication but root induction. Supplementing asparagus racemosus will aid digestion, since the plant has antiulcer effects. Propagation of asparagus racemosus through tissue culture. Rhizogenesis did not occur in individual shoots, upon their transfer to hormone free. In vitro propagation of wild asparagus adscendens roxb. Murashige and skoogs medium with 2, 4d and kinetin induced callus in the shoot segments of asparagus racemosus. Asparagus racemosus is an herb used in ayurveda medicine.
In indian system of medicine asparagus racemosus is an important medicinal plant and its root paste or root juice has been used in various ailments and as health tonic 1,2. The leaves and the tuberous roots of asparagus are medically important in several diseases. B adventitious shoot formation from surface of calli on ms with 2 mg l1 and 0. Asparagus racemosus, a climbing ayurvedic plant, is known for its numerous activities such as hyperlipidemia, hypertension, angina, dysmenorrhea, anxiety disorders, benign prostatic hyperplasia bph, leucorrhoea and urinary tract infections. The present study on micropropagation of asparagus racemosus through nodal explants showed better result in bap 2 mgl along. The majority of plants used by the pharmaceutical industry come from wild sources, endangering the natural population of the species. Somatic embryogenesis is one of the most popular in vitro regeneration methods for mass micropropagation. The succulent tuberous roots are 30100 cm long and 12 cm thick in bunch attached at the stem base. Manjeshwar shrinath baliga, in foods and dietary supplements in the prevention and treatment of disease in older adults, 2015. Safed musli ashwagandha shatavari churna hindi pdf herbal safedmusliashwagandhashat avarichurnahindi pdf.
Jan 11, 2011 a successful micropropagation system was developed for four different medicinal maesa species. Rapid in vitro protocol for high multiple shoot induction. Micropropagation of asparagus densiflorus via axillary shoots, indirect organogenesis and somatic embryogenesis anna pindel a. The majority of the compounds were produced by plants during different stages of cell, tissues and organ differentiation. Introduction asparagus racemosus have numerous medicinal usages which have been reported in the indian and british pharmacopoeias and in indigenous systems of medicine. In vitro propagation of spine gourd momordica dioica roxb. In some cases, tissue and cell culture is also used for the. In vitro propagation of four saponin producing maesa species. They reported that naa singly showed an increase in callus induction from nodes, internodes and shoot tips of asparagus racemosus. The demand for asparagus racemosus in 20012002 was 10,924. A induction of calli on shoot tips and nodal bud explants on ms medium supplemented with 4 mg l1 bap and 0. Review plant biotechnology, 152,51611998 somatic embryogenesis and its application for breeding and micropropagation in asparagus asparagus oflicinalis l. Plant tissue culture is the process by which parts of plant can be grown in vitro condition in a sterile culture medium. Asparagus racemosus supplement health benefits, dosage.
Family asparagaceae asparagus racemosus, commonly called satavar, shatavari, or shatamull, is native to the himalayas in india. Pdf micropropagation of asparagus by in vitro shoot culture. Posted on june 05, 2015 micropropagation of plants. Multiple shoots were induced through both axillary bud formation and adventitious shoot regeneration from leaf explants. Asparagus racemosus due to its slow growth faces lots of problem which can be solved through tissue culture. The asparagus racemosus shatavari or sanspayein is most commonly distributed species in tropical and subtropical regions of india. The present study on micropropagation of asparagus racemosus through nodal explants showed better result in bap 2 mgl along with iaa 0. Hisato kunitake and masahiro 201224 from the epidermal cells of explants or indirectly via intervening callus 4. The prodigious advantages of in vitro aseptic technique of clonal propagation i. Induction of somatic embryos in cultures of asparagus. Rooting was achieved with half strength ms basal medium plus iba. Asparagus racemosus is a valuable source of antioxidant and has significant cytotoxic activity hence could eliminate many diseases related to free radical. Shatavarin production from in vitro cultures of asparagus. Regeneration of asparagus racemosus by shoot apex and.
The multiple uses of this species have increased its commercial demand, resulting in overexploitation. Pdf asparagus officinalis is most extensively studied species within the genus asparagus, which is well known as garden asparagus. Asparagus racemosus is one of the important medicinal plants extensively used by the traditional practioners in india for its medicinal value. It grows 12 m 3 ft 3 in6 ft 7 in tall and prefers to take root in gravelly, rocky soils high up in piedmont plains, at 1,3001,400 m 4,3004,600 ft elevation. Radical scavenging, dpph, phenolic, flavonoid, cupric, reducing power. Ashtawarga plants suffering a triple standardization. Micropropagation of asparagus racemosus shatavari asparagus racemosus shatavari is a plant with immense medicinal values.
The plant occurs throughout india up to 1500 meters elevation. Asparagus racemosus a medicinally important plant can be propagated through tissue culture technique which save lots of time and money. Direct in vitro propagation of asparagus adscendens. Complete plants with cladode, crown and root systems were developed in hormone free. Regeneration of shoot buds and clonal multiplication of excised shoots through proliferation of nodal buds could be achieved by the use of iaa and bap in the medium. In vitro plantlet regeneration in asparagus racemosus through shoot bud differentiation on nodal segmentsashwani kumar and neetu vijay. Sterile cultures were obtained when the explants were treated with 0. Shatavari, micropropagation, tissue culture, in vitro culture. Asparagus racemosus wild asparagus racemosus willd. Sep 23, 2011 asparagus racemosus is an important monocot medicinal plant that is in great demand for its steroidal saponins called shatavarins. Investigation of cytotoxicity and invitro antioxidant. In vitro regeneration with callus development of asparagus. Asparagus officinalis as a valuable medicinal plant has a low multiplication rate using the conventional methods. In asparagus racemosus, there is an almost 100% mark up in price from the collector level to the user tewari, 2000.
Shatavari is a special female tonic because it helps women of all age groups to transit through natural. Asparagus racemosus in terms of qualitative and quantitative parameters of milk, in g 1 was control group, in which the animals receive experimental feed without any other feed supplements, whereas the animals of experimental group 2 g 2, group 3 g 3 and group 4 g 4 were fed a similar. P3007 development of a super spud through repeated cycles of marker free transformation. In vitro propagation of a high value medicinal plant. Because of the poor survival of the plantlets due to weak root formation, most of the micropropagation studies on asparagus have focused on the induction of somatic embryogenesis 46. All books are in clear copy here, and all files are secure so dont worry about it. Asparagus racemosus is one of the important medicinal plants found in india, china and other parts of the world. Efficient plant regeneration of asparagus by inducing normal. Asparagus adscendens, in vitro regeneration, multiple shoots, nodal explant. Three different media representing the whole micropropagation protocol of asparagus are described. The genus asparagus consisted of about 300 species around the world, out of which 22 species are recorded in india. Asparagus racemosus an overview sciencedirect topics. In wiley online library other applications of plant tissue culture which are not necessarily strictly propagation. An overview book pdf free download link or read online here in pdf.
Asparagus leaf extract inhibits growth of renal cell cancer cells asian pac j cancer prev, 15 5, 19371941 introduction asparagus racemosus willd. Pdf asparagus racemosus commonly known as shatavari in. Ayurveda is one of the oldest known holistic health care systems recommending diverse medicinal uses of plants for prevention and cure of diseases and illness. In vitro cellular and developmental biology plant bioone.
In vitro clonal propagation of asparagus racemosus, a high. Out of the 22 species of asparagus recorded in india. A method has been developed for rapid multiplication of curculigo orchioides hypoxidaceae through direct organogenesis and bulbil formation in vitro. This plant is known to produce steroidal saponins called shatavarins. P3026 the molecular events during the induction of shoot regeneration in plant tissue culture. Free aminoacids in the roots include aspartic acid, glycine, tyrosine, alanine, proline, tryptophan, glutamic acid and cystine. Asparagus racemosus is a well known ayurvedic rasayana which prevent ageing, increase longevity, impart immunity, improve mental function, vigor and add. Hair regrowth treatment provillus november 03, 2017. Mohommad shahid department of botany, davpg college, dehradun.
Leaf and underground stem explants produced maximum number of shoots four and 10 per explant, respectively on b5 medium supplemented with 4. Overview information asparagus racemosus is a plant used in traditional indian medicine ayurveda. It is reported to be recalcitrant to tissue culture practices. Phytochemical attributes of endemic endangered primrose. Pdf micropropagation and phytochemical analysis of. Study of anatomical biomarkers for the standardization of. Micropropagation of asparagus racemosus shatavari semantic. Neet biology strategies for enhancement in food production prepare. The plants are being overharvested, so this species faces a real danger of becoming. According to the website of american diabetes association type 2 diabetes is the most common form of diabetes, in type 2 diabetes, either the body does not.